PoplarV1, Main, Exploration, bibRecord, 000E03

Library screening of cell-penetrating peptide for BY-2 cells, leaves of Arabidopsis, tobacco, tomato, poplar, and rice callus.

Identifieur interne : 000E03 ( Main/Exploration ); précédent : 000E02; suivant : 000E04

Library screening of cell-penetrating peptide for BY-2 cells, leaves of Arabidopsis, tobacco, tomato, poplar, and rice callus.

Auteurs : Keiji Numata [Japon] ; Yoko Horii [Japon] ; Kazusato Oikawa [Japon] ; Yu Miyagi [Japon] ; Taku Demura [Japon] ; Misato Ohtani [Japon]

Source :

Scientific reports [ 2045-2322 ] ; 2018.

RBID : pubmed:30030484

Descripteurs français

KwdFr :
- Animaux (MeSH), Arabidopsis (composition chimique), Banque de peptides (MeSH), Feuilles de plante (composition chimique), Feuilles de plante (métabolisme), Lignée cellulaire (MeSH), Lycopersicon esculentum (composition chimique), Membrane cellulaire (métabolisme), Oryza (composition chimique), Peptides de pénétration cellulaire (analyse), Populus (composition chimique), Tabac (composition chimique), Techniques de transfert de gènes (MeSH).
MESH :
- analyse : Peptides de pénétration cellulaire.
- composition chimique : Arabidopsis, Feuilles de plante, Lycopersicon esculentum, Oryza, Populus, Tabac.
- métabolisme : Feuilles de plante, Membrane cellulaire.
- Animaux, Banque de peptides, Lignée cellulaire, Techniques de transfert de gènes.

English descriptors

KwdEn :
- Animals (MeSH), Arabidopsis (chemistry), Cell Line (MeSH), Cell Membrane (metabolism), Cell-Penetrating Peptides (analysis), Gene Transfer Techniques (MeSH), Lycopersicon esculentum (chemistry), Oryza (chemistry), Peptide Library (MeSH), Plant Leaves (chemistry), Plant Leaves (metabolism), Populus (chemistry), Tobacco (chemistry).
MESH :
- chemical , analysis : Cell-Penetrating Peptides.
- chemistry : Arabidopsis, Lycopersicon esculentum, Oryza, Plant Leaves, Populus, Tobacco.
- metabolism : Cell Membrane, Plant Leaves.
- Animals, Cell Line, Gene Transfer Techniques, Peptide Library.

Abstract

Cell-penetrating peptides (CPPs) are used for various applications, especially in the biomedical field. Recently, CPPs have been used as a part of carrier to deliver proteins and/or genes into plant cells and tissues; hence, these peptides are attractive tools for plant biotechnological and agricultural applications, but require more efficient delivery rates and optimization by species before wide-scale use can be achieved. Here, we developed a library containing 55 CPPs to determine the optimal CPP characteristics for penetration of BY-2 cells and leaves of Nicotiana benthamiana, Arabidopsis thaliana, tomato (Solanum lycopersicum), poplar (hybrid aspen Populus tremula × tremuloides line T89), and rice (Oryza sativa). By investigating the cell penetration efficiency of CPPs in the library, we identified several efficient CPPs for all the plants studied except rice leaf. In the case of rice, several CPPs showed efficient penetration into rice callus. Furthermore, we examined the relationship between cell penetration efficiency and CPP secondary structural characteristics. The cell penetration efficiency of Lys-containing CPPs was relatively greater in plant than in animal cells, which could be due to differences in lipid composition and surface charge of the cell membranes. The variation in optimal CPPs across the plants studied here suggests that CPPs must be optimized for each plant species and target tissues of interest.

DOI: 10.1038/s41598-018-29298-6
PubMed: 30030484
PubMed Central: PMC6054692

Affiliations:

Japon

Links toward previous steps (curation, corpus...)

to stream Main, to step Corpus: 000D37
to stream Main, to step Curation: 000D37

Le document en format XML

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<term>Arabidopsis (chemistry)</term>
<term>Cell Line (MeSH)</term>
<term>Cell Membrane (metabolism)</term>
<term>Cell-Penetrating Peptides (analysis)</term>
<term>Gene Transfer Techniques (MeSH)</term>
<term>Lycopersicon esculentum (chemistry)</term>
<term>Oryza (chemistry)</term>
<term>Peptide Library (MeSH)</term>
<term>Plant Leaves (chemistry)</term>
<term>Plant Leaves (metabolism)</term>
<term>Populus (chemistry)</term>
<term>Tobacco (chemistry)</term>
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<term>Arabidopsis (composition chimique)</term>
<term>Banque de peptides (MeSH)</term>
<term>Feuilles de plante (composition chimique)</term>
<term>Feuilles de plante (métabolisme)</term>
<term>Lignée cellulaire (MeSH)</term>
<term>Lycopersicon esculentum (composition chimique)</term>
<term>Membrane cellulaire (métabolisme)</term>
<term>Oryza (composition chimique)</term>
<term>Peptides de pénétration cellulaire (analyse)</term>
<term>Populus (composition chimique)</term>
<term>Tabac (composition chimique)</term>
<term>Techniques de transfert de gènes (MeSH)</term>
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<keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>Cell-Penetrating Peptides</term>
</keywords>
<keywords scheme="MESH" qualifier="analyse" xml:lang="fr"><term>Peptides de pénétration cellulaire</term>
</keywords>
<keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>Arabidopsis</term>
<term>Lycopersicon esculentum</term>
<term>Oryza</term>
<term>Plant Leaves</term>
<term>Populus</term>
<term>Tobacco</term>
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<keywords scheme="MESH" qualifier="composition chimique" xml:lang="fr"><term>Arabidopsis</term>
<term>Feuilles de plante</term>
<term>Lycopersicon esculentum</term>
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<term>Populus</term>
<term>Tabac</term>
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<keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Cell Membrane</term>
<term>Plant Leaves</term>
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<term>Membrane cellulaire</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Animals</term>
<term>Cell Line</term>
<term>Gene Transfer Techniques</term>
<term>Peptide Library</term>
</keywords>
<keywords scheme="MESH" xml:lang="fr"><term>Animaux</term>
<term>Banque de peptides</term>
<term>Lignée cellulaire</term>
<term>Techniques de transfert de gènes</term>
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<front><div type="abstract" xml:lang="en">Cell-penetrating peptides (CPPs) are used for various applications, especially in the biomedical field. Recently, CPPs have been used as a part of carrier to deliver proteins and/or genes into plant cells and tissues; hence, these peptides are attractive tools for plant biotechnological and agricultural applications, but require more efficient delivery rates and optimization by species before wide-scale use can be achieved. Here, we developed a library containing 55 CPPs to determine the optimal CPP characteristics for penetration of BY-2 cells and leaves of Nicotiana benthamiana, Arabidopsis thaliana, tomato (Solanum lycopersicum), poplar (hybrid aspen Populus tremula × tremuloides line T89), and rice (Oryza sativa). By investigating the cell penetration efficiency of CPPs in the library, we identified several efficient CPPs for all the plants studied except rice leaf. In the case of rice, several CPPs showed efficient penetration into rice callus. Furthermore, we examined the relationship between cell penetration efficiency and CPP secondary structural characteristics. The cell penetration efficiency of Lys-containing CPPs was relatively greater in plant than in animal cells, which could be due to differences in lipid composition and surface charge of the cell membranes. The variation in optimal CPPs across the plants studied here suggests that CPPs must be optimized for each plant species and target tissues of interest.</div>
</front>
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<Abstract><AbstractText>Cell-penetrating peptides (CPPs) are used for various applications, especially in the biomedical field. Recently, CPPs have been used as a part of carrier to deliver proteins and/or genes into plant cells and tissues; hence, these peptides are attractive tools for plant biotechnological and agricultural applications, but require more efficient delivery rates and optimization by species before wide-scale use can be achieved. Here, we developed a library containing 55 CPPs to determine the optimal CPP characteristics for penetration of BY-2 cells and leaves of Nicotiana benthamiana, Arabidopsis thaliana, tomato (Solanum lycopersicum), poplar (hybrid aspen Populus tremula × tremuloides line T89), and rice (Oryza sativa). By investigating the cell penetration efficiency of CPPs in the library, we identified several efficient CPPs for all the plants studied except rice leaf. In the case of rice, several CPPs showed efficient penetration into rice callus. Furthermore, we examined the relationship between cell penetration efficiency and CPP secondary structural characteristics. The cell penetration efficiency of Lys-containing CPPs was relatively greater in plant than in animal cells, which could be due to differences in lipid composition and surface charge of the cell membranes. The variation in optimal CPPs across the plants studied here suggests that CPPs must be optimized for each plant species and target tissues of interest.</AbstractText>
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<ForeName>Keiji</ForeName>
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<Author ValidYN="Y"><LastName>Oikawa</LastName>
<ForeName>Kazusato</ForeName>
<Initials>K</Initials>
<AffiliationInfo><Affiliation>Biomacromolecules Research Team, RIKEN Center for Sustainable Resource Science, 2-1 Hirosawa, Wako-shi, Saitama, 351-0198, Japan.</Affiliation>
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<Author ValidYN="Y"><LastName>Demura</LastName>
<ForeName>Taku</ForeName>
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<AffiliationInfo><Affiliation>Biomacromolecules Research Team, RIKEN Center for Sustainable Resource Science, 2-1 Hirosawa, Wako-shi, Saitama, 351-0198, Japan.</Affiliation>
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<AffiliationInfo><Affiliation>Graduate School of Biological Sciences, Nara Institute of Science and Technology, Ikoma, Nara, 630-0192, Japan.</Affiliation>
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<AffiliationInfo><Affiliation>Graduate School of Biological Sciences, Nara Institute of Science and Technology, Ikoma, Nara, 630-0192, Japan.</Affiliation>
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<Agency>Japan Science and Technology Agency (JST)</Agency>
<Country>International</Country>
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<Month>07</Month>
<Day>20</Day>
</ArticleDate>
</Article>
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<ISSNLinking>2045-2322</ISSNLinking>
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<QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName>
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<MeshHeading><DescriptorName UI="D002460" MajorTopicYN="N">Cell Line</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D002462" MajorTopicYN="N">Cell Membrane</DescriptorName>
<QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D057846" MajorTopicYN="N">Cell-Penetrating Peptides</DescriptorName>
<QualifierName UI="Q000032" MajorTopicYN="Y">analysis</QualifierName>
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<MeshHeading><DescriptorName UI="D018014" MajorTopicYN="Y">Gene Transfer Techniques</DescriptorName>
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<MeshHeading><DescriptorName UI="D018551" MajorTopicYN="N">Lycopersicon esculentum</DescriptorName>
<QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D012275" MajorTopicYN="N">Oryza</DescriptorName>
<QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName>
</MeshHeading>
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Library screening of cell-penetrating peptide for BY-2 cells, leaves of Arabidopsis, tobacco, tomato, poplar, and rice callus.

Library screening of cell-penetrating peptide for BY-2 cells, leaves of Arabidopsis, tobacco, tomato, poplar, and rice callus.

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